To conclude, although our study has not identified a direct role of NPHP1 and NPHP4 in regulation of cholesterol content in mIMCD3 cells exposed to shear stress, we showed that targeting the cholesterol pathway in KD_N1 and KD_N4 cells influences cilium length regulation, potentially offering a new therapeutical approach for NPH patients. Here, NPHP1 is linked to normal pressure hydrocephalus.