Combine single-cell RNA sequencing along with spatially resolved transcriptomic sequencing to gain spatial insights into the complex crosstalk, cellular states, and cellular plasticity leading to the immunosuppressive environment found in GBM. Spatial transcriptomics was used to identify the spatial overlap of cells; was observed that the HMOX1+ myeloid cells were spatially correlated with T cell exhaustion and the mesenchymal state of glioblastoma. Also confirmed the presence of HMOX1+ myeloid cells to result in the reduction of the effector t cell population. This evidence concerns the gene HMOX1 and glioblastoma.