Four potential VP343 targets (i.e., Mvk, Scarb2, Pik3c3, or Sirt2), identified by proteomic analysis, were knocked down by transfection of macrophages with specific siRNA prior to their infection with L. infantum in order to determine their role in parasite survival, Lamp-1 recruitment, and thus in the mechanism of action of VP343. This evidence concerns the gene PIK3C3 and infection.