Regarding the association between FAM171B and the macrophage phenotype in BLCA, immunoassays based on the QUANTISEQ algorithm revealed a significant positive correlation between FAM171B expression and M2 macrophages (Fig. 1P), while no significant correlation with M1 macrophages was observed (Fig. 1O). The gene discussed is FAM171B; the disease is bladder transitional cell carcinoma.