Similar to myeloid cells, we observed that the GnRH signaling pathway and ErbB signaling pathway pathway were upregulated in these two HCC associated CD8+ T cell clusters (Fig. 8E), however, both signaling pathways are downregulated in HCC-related NKT cell (Supplementary Fig. 1E). The gene discussed is GNRH1; the disease is hepatocellular carcinoma.