RT-qPCR confirmed overall upregulation of a subset of NASH progression signature genes35 in Fbxw7-null livers, including Akr1b10, Ccl20, Clic6, Col1a1, Col1a2, Epb41l4a, Fermt1, Gdf15, Itgbl1, Ltbp2, and Thy1 (Fig. 1c). This evidence concerns the gene GDF15 and metabolic dysfunction-associated steatohepatitis.