To confirm the improved penetration of immune cells in tumors, we performed flow cytometry analysis of total immune cells (CD45+ cells), helper T lymphocytes (CD45+CD3+CD4+ cells) and CTL (CD45+CD3+CD8+ cells) in 4T1-tumors (Fig. S18) and in RIL-175 HCC (Fig. S19). The gene discussed is CD8A; the disease is hepatocellular carcinoma.