To determine how fast AAV-Cacna2d1 viral infection can increase the expression level of CACNA2D1 in DIO mice, we explored the mRNA levels of Cacna2d1 1 day and 3 days after bilaterally injecting AAV-DIO-Cacna2d1 into the vlPAG brain region of DIO Gad2-Cre mice by RNA scope. The gene discussed is GAD2; the disease is viral infectious disease.