Additionally, we have performed cytotoxicity for anticancer phytochemicals including topotecan, and brusatol on these breast cancer cell lines (Fig 9) subsequently the selected concentrations were used for treatment onto BT-474, T-47D cell lines for Western blotting to examine the expression patterns of FN3K, Nrf2 antioxidant downstream signaling proteins. Here, FN3K is linked to breast cancer.