As ECs and IELs both express genes for AMP proteins, e.g., Reg3γ,41,42 we separated ECs from IELs using Percoll density gradients, as in recent reports.43,44 We found that young male mice increased the mRNA expression of multiple Reg3 genes at day 7 post-stroke compared to young sham mice (Figure 1b). Here, REG3G is linked to Stroke.