At 20 h post infection (hpi), the percentage of the parasitophorous vacuoles (PVs) containing one parasite in the control group was much higher than that in the Cbl-b KD group (P < 0.05), while the percentage of the PVs with four parasites in the control group was much lower than that in the Cbl-b KD group (P < 0.05), and no significant difference was found for the percentage of the PVs with two parasites between these two groups (Fig. 1B). Here, CBLB is linked to infection.