PROS1 and infection: However, it is worth noting that the significant increase of Drp1 phosphorylation was shown at 2 h post-infection and at 12 h post-transfection, where NS1 expression appears to be quite low, we cannot rule out the possibility that other factors, such as other viral structural proteins, virus adsorption process, or the stress stimuli of transfection, may increase the Drp1 phosphorylation.