By further examining changes in the PI3K/AKT/mTOR pathway looking at the downstream transcription factors S6K and eIF4E, and PRAS40 (inhibits mTOR and IRS-1), we could identify their role in the negative feedback of the PI3K pathway, an effect commonly seen in various cancers and cancer models (27, 34–36). This evidence concerns the gene AKT1 and cancer.