The human recombinant IgG1, C4, targeting human and murine PD-L1 and its engineered low FcRn affinity (H310A/H435Q) mutant (C4Fc-MUT) were used to perform FUS-aided immunoPET in a syngeneic glioblastoma murine model (Scheme 1). This evidence concerns the gene CD274 and glioblastoma.