Immunohistochemical studies consistently demonstrated weak Tet1 staining in renal glomeruli and tubular cells under non-injury conditions; after I/R injury, a significant increase in the nuclear Tet1 level was found in renal tubules and glomeruli at the AKI stage and the AKI to CKD stage with no gender-based differences (Figure S1A-B). This evidence concerns the gene TET1 and chronic kidney disease.