There is an emerging technique called “sonobiopsy” to facilitate the ctDNA release in the blood with the means of sonication disruption of tumor cells and blood-CSF barrier, which was shown to increase the sensitivity of the glioma marker DNA sequence alteration detection for EGFRvIII and p-TERT SNVs (Pacia et al., 2022). This evidence concerns the gene TERT and central nervous system cancer.