After adjusting for clinical variables, including locational information, age, tumor basal diameter, sex, histopathology, and tumor stage, multivariate Cox regression analysis revealed that high infiltration of MΦ-C4 remained significantly associated with poor survival in four UM cohorts (TCGA: HR 57, 95% CI 9.9–320, P < 0.001; Laurent’s: HR 3.7, 95% CI 1.7–8.4, P = 0.0015; Gangemi’s: HR 18, 95% CI 3.2–100, P = 0.0011; van Essen’s: HR 4, 95% CI 0.89–18, P = 0.072) (Supplementary Fig. 5). This evidence concerns the gene C4A and neoplasm.