CXCL8 and intrahepatic cholangiocarcinoma: It was found that higher expression of IL-8 or CD97 was associated with shortened 5-year survival rates, as shown in Fig. 1E. Finally, IL-8 at 25, 50, 100, and 200 ng/mL was utilized in QBC-939 and RBE ICC cell lines, and cell lysates were collected after 24 h or 48 h of treatment.