In the early-onset T1D model, this dual-anchor coupling approach exhibited effective immunoregulation by reducing the infiltration of IFN-γ and GzmB producing (CD3+CD8+IFN-γ+ and CD3+CD8+GzmB+) cytotoxic T lymphocytes and recruiting more CD3+CD4+FoxP3+ Tregs, thereby reversing 57.1% of newly hyperglycemic NOD mice after 100 days. Here, GZMB is linked to type 1 diabetes mellitus.