In the group of treating with donor supernatant from ZZCD group, the depressive behaviors were decreased in SPT (p < 0.01) and FST (p < 0.001), anxiety behaviors were decreased in EPM (p < 0.01) and OFT (p < 0.001), and the levels of 5‐HT, GABA (both p < 0.01) and IL‐10 (p < 0.05) were increased and the level of IL‐1β (p < 0.01) was decreased. The gene discussed is IL1B; the disease is Anxiety.