According to results of ELISA, ZZCD can exert antidepressant and anxiety effects by reducing the levels of pro‐inflammatory factors (IL‐1β and TNF‐α) in peripheral blood, increasing the levels of anti‐inflammatory factors (IL‐10) and neurotransmitters (5‐HT, DA and GABA), and regulating HPA axis hormones (CRH, ACTH, and CORT). This evidence concerns the gene CRH and Anxiety.