In our study, we observed that NLRP3 inflammasome was the effector of the KLF9/miR‐494‐3p/PTEN axis and activation of NLRP3 using nigericin reversed the alleviative role of silencing KLF9 in CSE‐induced inflammation, suggesting the involvement of the KLF9/miR‐494‐3p/PTEN axis in NLRP3 inflammasome‐mediated airway inflammation. The gene discussed is NLRP3; the disease is inflammatory response.