Next, we performed the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis on these target genes, and we demonstrated that the functional HRR-associated enhancers revealed by our CRISPRi screen can drive tumor growth through several canonical cancer pathways, such as the EGFR signaling pathway, central carbon metabolism, and AMPK signaling pathway (Fig. 4b). This evidence concerns the gene EGFR and neoplasm.