As expected, we acquired the similar phenotypes with the E_156 inhibition (all p-value < 0.01) (Additional file 1: Fig. S9q, s); when E_156 core region was deleted, the interaction between PTEN promoter and the region harboring E_156 was lost (Additional file 1: Fig. S8g), further validating the E_156 directly regulates PTEN expression in melanoma growth. Here, PTEN is linked to melanoma.