In prostate cancer, GAD1 expression was significantly positively correlated with Tem CD8 (r = 0.251), Th1 (r = 0.241), CD56dim (r = 0.252), MDSC (r = 0.248) and Macrophage (r = 0.243) (Fig. 5A); GAD1 methylation was positively correlated with Tem CD4 (r = 0.278), Th2 (r = 0.258), NK (r = 0.268) and iDC (r = 0.278), where Macrophage was not significantly correlated (r = 0.143) (Fig. 5A). This evidence concerns the gene CD4 and prostate cancer.