PSMA and GAD1 were key enzymes in glutamate production and metabolism, respectively, and it is worthwhile to explore thoroughly whether GAD1 had important functions and specificity similar to PSMA. Therefore, this study was the first to propose and initially demonstrate through in vitro experiments that GAD1 influenced the progression, metastasis, and drug sensitivity of prostate cancer, which predicted an important physiological or pathological function of GAD1 in prostate cancer. This evidence concerns the gene GAD1 and prostate carcinoma.