In the TCGA-PRAD cohort, GAD1 single gene differential analysis combined with GSEA enrichment analysis revealed that pathways mainly associated with multiple immune-related pathways, such as B cell receptor, nuclear factor kappa B (NFkB) activation, interleukin (IL)-4 and IL-13, T cell receptor, integrin cell surface interactions, etc (Fig. 6A). Here, IL13 is linked to prostate adenocarcinoma.