Finally, with the help of the human protein atlas database (HPA) database (https://www.proteinatlas.org), GAD1 expression was confirmed to show an increasing trend in prostate tissue (Fig. 7N), high-grade prostate cancer (Fig. 7O), as well as low-grade prostate cancer (Fig. 7P), which was consistent with GAD1 expression in different grade (Fig. 3F). The gene discussed is GAD1; the disease is prostate carcinoma.