In this study, bioinformatic and prognostic analyses of the GSE33455, GSE36135, GSE78201, GSE104935, and GSE143408 datasets from prostate cancer cell lines were used to identify Glutamate decarboxylase 1 (GAD1), which as a potential key gene affecting the prognosis, progression, and drug resistance of prostate cancer. Here, GAD1 is linked to prostate cancer.