Interestingly, in the present study we found that L. casei & L. reuteri inhibited the expression of TLR4 and MyD88 in pancreatic cancer cells, and TLR4 inhibitors displayed similar anti-pancreatic cancer effects as L. casei & L. reuteri, which suggested that L. casei & L. reuteri exerted their suppressive effects on pancreatic cancer by inhibiting TLR4. This evidence concerns the gene TLR4 and pancreatic neoplasm.