To predict the potential mechanism, we carried out GSEA to select KEGG pathways influenced by NR6A1, OSBP2 and UNC119B. As shown, NR6A1, OSBP2 and UNC119B all contributed to the activation of the KEGG-WNT-signaling-pathway and the KEGG-MAPK-signaling-pathway, suggesting that these two pathways might be activated by histone Kla in HCC, and contribute to therapy resistance (Fig. 6A–C, Table 2). The gene discussed is NR6A1; the disease is hepatocellular carcinoma.