Upon transduction of PDK4 into PCa lines, we observed significantly enhanced expression of a subset, albeit not all SASP factors, as well as senescence markers p16INK4a and p21CIP1 in cells overexpressing PDK4 and treated by MIT (compared to cells transduced with vector and damaged by MIT; Supplementary Fig. 5a–c). This evidence concerns the gene CDKN1A and posterior cortical atrophy.