We performed RNA-seq variant calling to detect the most relevant and replicated single-nucleotide polymorphisms (SNPs) identified as risk modifiers of MASLD progression (rs738409, rs72613567, rs58542926 and rs641738 in the genes patatin-like phospholipase domain containing 3 (PNPLA3), hydroxysteroid 17-beta dehydrogenase 13 (HSD17B13), transmembrane 6 superfamily member 2 (TM6SF2) and membrane-bound O-acyltransferase domain containing 7 (MBOAT7), respectively). The gene discussed is HSD17B13; the disease is metabolic dysfunction-associated steatotic liver disease.