TP53 and hepatocellular carcinoma: mRNA profiling of the HB and HCC-like components was performed using a custom 635-gene NanoString expression panel designed to profile pediatric liver neoplasms (HB and HCC) cancer pathway activation, including WNT pathway genes, p53 activity, MAPK signaling, and oxidative stress, as well as housekeeping genes (Supplemental Table S2).