In addition, we also observed that after blocking PlexinB1, Sema4A-induced proliferative, migratory and invasive abilities as well as viability of LC cells were suppressed evidently, but blocking PlexinB2 and PlexinD1 did not always significantly inhibit Sema4A-induced proliferative, migratory and invasive abilities as well as viability for LC cells, these observations implicated that Sema4A might contribute to the development of LC by interacting with PlexinB1. The gene discussed is SEMA4A; the disease is laryngotracheoesophageal cleft.