To clarify the molecular mechanism of Sema4A in the development of LC, we stimulated NCI-H460 cells with PlexinB1 blocking antibody, 100 nmol/L of Sema4A-Fc protein, alone or in combination, and then detected the expressions of proteins associated with the NF-κB pathway. Here, NFKB1 is linked to laryngotracheoesophageal cleft.