(46) found that sublethal doses of pemetrexed (100nM) and 5-fluorouracil (5-FU) could upregulate PD-L1 expression and regulate TIL activity in NSCLC cells, and found that pemetrexed or 5-FU elevated PD-L1 protein levels in a dose-dependent manner. This evidence concerns the gene CD274 and non-small cell lung carcinoma.