AKT1, TP53, ESR1, SRC, and EGFR had energy <−5 kcal/Mol, demonstrating that these five core targets of AE play an important role in the treatment of HCC(Li et al., 2021; Liu et al., 2021; Li T. et al., 2022). This evidence concerns the gene ESR1 and hepatocellular carcinoma.