The results showed that AKT serine/threonine kinase 1 (AKT1), TP53, albumin (ALB), estrogen receptor 1 (ESR1), TNF, signal transducer and activator of transcription 3 (STAT3), caspase 3 (CASP3), epidermal growth factor receptor (EGFR), and SRC proto-oncogene (SRC) may be the key factors in the treatment of HCC using AE. The gene discussed is ESR1; the disease is hepatocellular carcinoma.