Since we observed that aggressive PCa cell lines are highly methylated at SPDEF cis-regulatory elements, we hypothesized that blocking DNA methylation via DNA methyltransferase inhibitor, 5-Aza-2-deoxycytidine (5-Aza-dC) might be able to mimic the phenotypic changes similar to those we observed in RC77/T-SPDEF overexpression. Here, SPDEF is linked to posterior cortical atrophy.