After HFD feeding, NAFLD rats showed significantly increased body weight (P = 0.0056, Figure 2A), liver weight (P < 0.0001, Figure 2B), liver/body ratio (P = 0.0003, Figure 2C), HOMA-IR index (P = 0.0135, Figure 2F), serum ALT (P = 0.0018, Figure 2G), and hepatic TG (P = 0.0005, Figure 2H) compared with rats in the Control group. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.