Consistent with the flow cytometry data, quantitative PCR revealed that the transcript levels of Cd80 and Inos, markers for M1 macrophage, and the transcript levels of cytokines Tnfα, Il1β, Ifnγ, and Il17 were increased in lungs from neutrophilia-dominant mice compared to control and eosinophilia-dominant mice, but were decreased after rmIL-25 treatment (Fig. 2D and I). This evidence concerns the gene CD80 and Increased total eosinophil count.