Prognostic analysis revealed that 42 DEGs were significantly associated with the OS of AML patients (Fig. 10A, Table S10), and the top three significant DEGs were AC092042.3 (adjusted P < 0.0001, adjusted HR = 0.352, 95% CI 0.219–0.566, Fig. 10B), matrix metallopeptidase 7 (MMP7, adjusted P < 0.0001, adjusted HR = 2.761, 95% CI 1.726–4.415, Fig. 10C), and SIX homeobox 3 (SIX3, adjusted P < 0.0001, adjusted HR = 0.374, 95% CI 0.234–0.598, Fig. 10D). The gene discussed is SIX3; the disease is acute myeloid leukemia.