Indeed, the cytotoxic activity of the ADC was tested by MTT assay after 5 days exposure, and IC50 values (0.01, 0.39, and 4.7 nM for CRC cell lines expressing higher levels of HER-3) clearly showed that the ADC induces a potent cell-killing activity in a target dependent fashion (Fig. 3). The gene discussed is ERBB3; the disease is colorectal carcinoma.