Given the association of SPEM with intestinal-type rather than the diffuse-type of GC in humans, and to substantiate the striking correlation between expression signatures and patient outcomes for intestinal-type GC, we used multiplex immunohistochemistry on tissue microarrays of intestinal-type GC to identify GATA3+CD3- ILC2s and choline O-acetyltransferase (ChAT+) tuft cells, respectively57,60. The gene discussed is CHAT; the disease is gastric cancer.