Remarkably, when GAL-3 was silenced or overexpressed in ovarian cancer cells, there was a decrease or increase in NICD1 cleavage, respectively, as well as changes in the expression of the NOTCH target genes Hes1 and Hey1. Additionally, by co-immunoprecipitation, GAL-3 CRD was demonstrated to interact with NOTCH-1 and NICD1, and GAL-3 overexpression increased NICD1 nuclear translocation (117). This evidence concerns the gene LGALS3 and ovarian cancer.