Results in Figure 7A showed that the nsp3 was detected as early as 4 h.p.i., and the signal was increased along the infection time course, revealing the initial translation occurred at approximately 4 h.p.i. The MG132 treatment greatly suppressed the synthesis of nsp3 in both Vero and DF-1 cells, indicating that UPS affects initial translation. The gene discussed is SH2D3C; the disease is infection.