In this study, the nanobubbles served as a carrier to deliver a mixture of Cathepsin K (CTSK) siRNA and cerium oxide nanoparticles (CeNPs) to inhibit CTSK secretion by osteoclast cells, which triggers osteoporosis and enhances mineralization with antioxidant properties of CeNPs. The gene discussed is CTSK; the disease is osteoporosis.