In an invasion assay conducted using a transwell plate coated with matrix gel, we found that the supplementation of MMP1 or simultaneous supplementation of MMP1 plus MMP3 significantly reversed the inhibitory effect of Cant on RA-FLS cell vertical migration, while the supplementation of MMP3 alone did not have this effect (Figure 3B,D). This evidence concerns the gene MMP1 and rheumatoid arthritis.