By Western blot, ELISA, and qRT-PCR analysis, we found that Cant treatment dramatically reduced the production of MMP1 and MMP3, but not MMP2, in TNF-α-stimulated RA-FLSs, indicating that one of the ways by which Cant regulates the aggressive behavior of RA-FLSs is by reducing their production of MMP1 and MMP3. This evidence concerns the gene MMP1 and rheumatoid arthritis.