In vitro efficacy assays were also performed, in which the developed niosomes achieved a higher acetylcholinesterase and free radical (2,2-diphenyl-1-picrylhydrazyl) formation inhibition than the drug solution, showing relevant anticholinesterase and antioxidant properties, which are both quite relevant in Alzheimer’s disease pathophysiology. This evidence concerns the gene ACHE and Alzheimer disease.