We investigated the transcriptional state of the NF-κB pathway in a panel of ALL cell lines, corroborating in vitro the findings on patients’ gene expression data, and generated stable transduced ALL cell lines expressing an NF-κB reporter construct that incorporated a copy of the firefly luciferase gene containing an NF-κB binding site within its promoter region. The gene discussed is NFKB1; the disease is acute lymphoblastic leukemia.