By performing a GSEA on publicly available gene expression data from bone-marrow-derived blasts from 161 newly diagnosed pediatric ALL patients, we identified constitutive TNF-α signaling via NF-κB pathway as the most enriched Cancer Hallmark among patients with higher WBC counts following a single-agent-based frontline therapy utilizing MTX. Here, NFKB1 is linked to acute lymphoblastic leukemia.