After 6 days, mice were divided into ten groups according to stratified randomization based on tumor size to make sure that each group has similar average tumor size, and each group of mice was given a different treatment (group 1: control; group 2: TC-1; group 3: TC-1 + Ad-control; group 4: TC-1 + Ad-E7; group 5: TC-1 + PD-1 antibody; group 6: TC-1 + IgG 2a; group 7: TC-1 + PD-L1 antibody; group 8: TC-1 + IgG 2b; group 9: TC-1 + Ad-E7 + PD-1 antibody; group 10: TC-1 + Ad-E7 + PD-L1 antibody). This evidence concerns the gene PDCD1 and neoplasm.