In this study, an Ampiseq targeted NGS panel and Illumina TruSeq WES were both used to pinpoint six specific genes (KIF6, DRC7, STPG2, SPATA16, CFTR, CMTM2) that play a role in MT association and spermiogenesis, which are crucial factors in understanding the causes of male infertility. Here, STPG2 is linked to male infertility.