In this study, an Ampiseq targeted NGS panel and Illumina TruSeq WES were both used to pinpoint six specific genes (KIF6, DRC7, STPG2, SPATA16, CFTR, CMTM2) that play a role in MT association and spermiogenesis, which are crucial factors in understanding the causes of male infertility. This evidence concerns the gene SPATA16 and male infertility.