The differential proteins HMOX1, TFR1 and SLC3A2, which are associated with stroke by regulating the iron death pathway after the action of Iristectorin B, were identified using TMT relative quantification combined with PRM targeted validation technology, and the overall trend of the PRM and Western blotting validation results and the results of the TMT experiments were of high consistency. The gene discussed is SLC3A2; the disease is Stroke.