Specifically, 5-Aza activated TUSC3 expression in U251 GBM cells (MGMT-hypermethylated) and further decreased TMZ IC-50 (Figure S8A,B), while 5-Aza and Lomeguatrib combination successfully activated TUSC3 in T98G GBM cells (MGMT-hypomethylated) and sensitized cells to TMZ treatment (Figure S8A,B). The gene discussed is MGMT; the disease is glioblastoma.