Furthermore, by using live cell imaging of live-stained VE-cadherin-GFP junctions of the pMBMECs and restriction of melanoma cells adhesion to flow-resistant events, we advance knowledge by temporally resolving melanoma cell extravasation and confining extravasation events to those melanoma cells capable of adhering to the pMBMECs under blood flow conditions. Here, CDH5 is linked to melanoma.