Therefore, we conclude that the increased intercalation of the YUMM1.1-BrM4 melanoma cells into the PECAM-1-ko pMBMECs compared to the PECAM-1-wt pMBMECs is solely caused by the compromised endothelial cell junctions and is independent of an inflammatory state of the pMBMECs or increased melanoma cell adhesion. This evidence concerns the gene PECAM1 and melanoma.