In order to investigate the possible contribution of AKT isoforms to metastatic potential in human cell lines, we generated luciferized doxycycline-inducible shRNA hairpins to AKT1, AKT2, and AKT3 [34], as well as a nontargeting hairpin (shNT) that efficiently reduced protein expression in the majority of melanoma cell lines from our panel, (Figure 1A,B and Figure S1C–E) without affecting viability (Figure 1C). Here, AKT1 is linked to melanoma.