AKT1 and metastatic neoplasm: While AKT2 depletion after metastatic seeding significantly improved overall survival in the doxycycline chow-fed mice versus regular chow mice, the doxycycline-fed mice did succumb to eventual metastatic disease, suggesting that AKT2 reduction only delayed the growth of the metastatic lesions, and other AKT activity might compensate over the long term (Figure 3E).