In order to further investigate the impact of the genetic loss of each AKT isoform on melanoma progression, we crossed melanoma-prone BRAFV600E; Arf−/− mice with AKT isoform knockout mice [23] to generate a BRAFV600E; Arf−/−; AKT−/− compound mutant mouse (Breeding scheme; Supplementary Figure S4D). This evidence concerns the gene CDKN2A and melanoma.