NKX3.2 expression between normal and tumor tissues significantly differs in breast invasive carcinoma (p < 0.05), bladder urothelial carcinoma (p < 0.001), cholangiocarcinoma (p < 0.001), glioblastoma multiforme (p < 0.001), head and neck squamous carcinoma (p < 0.001), lung squamous cell carcinoma (p < 0.001), lung adenocarcinoma (p < 0.001), rectal cancer (p < 0.05), and LIHC (p < 0.001) (Figure 1A). This evidence concerns the gene NKX3-2 and cholangiocarcinoma.